IADR Abstract Archives

Accelerated oral wound healing using a pre-vascularized mucosal cell sheet

Recent trends and advances of newly developed, tissue engineered scaffold, calledpre-vascularized mucosal cell sheets and the effect of the sheets on wound healing in oral cavity. Mucosal keratinocytes, fibroblasts, and endothelial progenitor cells were primarily cultured for in vitro cell expansion from mucosa and blood of SpragueDawley rats. Mucosal cell sheets were generated using cultured keratinocytes and plasma fibrin (K sheet) or keratinocytes and a mixture of fibrin, fibroblasts, and endothelial cells (PV sheet). Autologous sheets were transplanted on deep wounds in the buccal region of rats. The gross and histological characteristics of wound healing were compared among control wound, K sheet, and PV sheet groups. We successfully cultured and expanded keratinocytes, fibroblasts, and endothelial progenitor cells in vitro for generating mucosal cell sheets with or without pre-vascularization. In the in vivo oral wound model, compared with the control wound, the PV sheet group exhibited rapid wound closure more prominently than the K sheet group. The histological healing in the PV sheet group was similar to that in rat normal buccal mucosa without fibrosis. The pre-vascularized mucosal cell sheet exhibited in vivo efficacy in oral wound healing by promoting accelerated healing.
Iranian Division Meeting
2019 Iranian Division Meeting (Tehran, Iran)
Tehran, Iran
2019

Accepted Abstracts
  • Sharifzadeh, Saba  ( D.D.S. Dentist, resident of oral medicine,.Membership of Dental Material Research Center, Tehran Medical Science, Islamic Azad University, Tehran, Iran )
  • Alaee, Arezoo  ( D.D.S.Dentist ,specialist of oral medicine,.Membership of Dental Material Research Center, Tehran Medical Science, Islamic Azad University, Tehran, Iran )
  • Eskandarion, Solmauz  ( D.D.S. PhD, Dental Material Research Center, Tehran Medical Science Islamic Azad University )
  • None
    None
    Poster Presentation