Methods: 20 samples (newly extracted intact premolars) were included in this experimental study. They were divided into two groups of 10. First the enamel roughness of each group of samples was measured before treatment by profilometer device (RT200 Roughness profilometers, USA ).Then one group was bleached twice daily for 14 days and each time for 1 h by Opalescence (Ultradent, USA) 22%CP and the other group was bleached by Whiteness Perfect (FGM, Brazil) 20%CP. Immediately after bleaching, the enamel roughness of each sample was measured again. The samples were immersed in artificial saliva throughout the study. After bleaching, fluoride (Flor Opal1.1%) was applied on each sample for 8 hours and the enamel roughness of each sample was measured. One Way ANOVA was used to compare the difference between groups.
Results: In both groups after bleaching the enamel roughness was increased and the enamel roughness was decreased after fluoride therapy and it was even lower than the enamel roughness before treatment. There was no significant difference between these two groups.
Conclusions: According to this study, it was shown that bleaching will increase the enamel roughness but artificial saliva and fluoride therapy after bleaching not only remove the harmful effects but also improve the enamel roughness even before than baseline.