Methods: The samples (n=24) of Wistar albino rats weighing 250±30 g received subcutaneously polyethylene tubes filled with biodentine and MTA which were mixed by titration and conventional technique. 15, 30, and 60 days after implantation, the animals were sacrificed and specimens were prepared for microscopic analysis. The setting times of cements were analyzed using Gilmore needles according to the ISO 6876 specification. Data were analyzed by ANOVA and Tukey's test (P≤0.05) for setting time and Kruskal-Wallis and Dunn test for biocompatibility.
Results: Histologic observation of dental cements 30 and 60 days revealed no statistically significant difference (P>0.05) among the materials in the subcutaneous tissues but it was significant on day 15. The initial setting time for MTA and Biodentine regarding the method of mixing (40, 32 min) were (28, 12min) for MTA and biodentine respectively.
Conclusions: All the cements represent good histocompatibility but it seems that the method of mixing may improve this initial setting time so may have influenced the release of some ingredients of cements like Ca+ and physiological characteristics.