IADR Abstract Archives

The Osteoregenerative Effects of PDGF-BB with MSCs Loaded on FDBA

Objectives: Due to shortcomings associated with autogenous bone graft, the gold standard of craniofacial grafting, investigators seek alternatives that are accessible, efficient, and affordable. Accordingly, in the present pilot study, bone regeneration was induced using Bone Marrow derived Mesenchymal Stem Cells (BMCS) loaded onto Freeze Dried Bone Allograft (FDBA) blocks in the presence or absence of recombinant platelet-derived growth factor-BB (rhPDGF-BB). Treatments were tested on critical sized defects created in canine mandible.

Methods: 8 weeks after the bilateral extraction of premolars of 4 mongrel dogs, 25×10 mm defects were created at both sides of the mandible. The right mandible received autogenous-BMSC loaded on FDBA (control), whereas the left mandible received cellular blocks impregnated with rhPDGF-BB (experimental). Animals were euthanized 8 weeks after grafting. Mandibles were harvested and transferred to the laboratory for micro-computed tomography (micro-CT) and histomorphometric analysis. The Mann-Whitney test was used for data analysis and statistical significance was set at P<0.05.

Results: Although differences were not statistically significant, micro-CT and histomorphometric analysis demonstrated higher levels of bone formation for the test group (5.34%±0.20 and 16.63%±3.14 respectively) when compared to the control group (4.20%±0.20 and 11.38%±5.11).

Conclusions: According to the performed micro-CT and histomorphometric analysis, adding 0.5 mg rhPDGF-BB (0.3 mg/ml) to the combination of BMSC/FDBA might have amplified bone formation in critical sized defect in dog mandible.


Iranian Division Meeting
2013 Iranian Division Meeting (Tehran, Iran)
Tehran, Iran
2013

Scientific Program
  • Khojasteh, Arash  ( Assistant Professor of Oral and Maxillofacial Surgery Shahid Beheshti University of Medical Sciences, Tehran, , Iran )
  • Dashti, Seyedeh Ghazaleh  ( Doctor of Dental Surgery, Dental Research Center, Research Institute of Dental Sciences of ShahidBeheshti University of Medical Sciences, tehran, , Iran )
  • Dehghan, Mohammad Mehdi  ( Veterinary Surgeon, Associate Professor, Department of Surgery and Radiology,Center of Excellence for Cell Therapy and Tissue Engineering,Faculty of Veterinary Medicine, University of Tehran, tehran, , Iran )
  • Kheiri, Lida  ( Student, Gifted and Talented Dental Students Division, Dental School, Shahid Beheshti University of Medical Sciences, tehran, , Iran )
    • Poster Session
    Poster 4
    12/12/2013