Interleukin-10 Gene Polymorphisms in Patients with Recurrent Aphthous Stomatitis
Objectives: Recurrent aphthous stomatitis (RAS) is a common oral inflammatory disease with unknown etiology in which the immune system seems to have a role in oral tolerance. Interleukin (IL)-10 is a cytokine synthesis inhibitory factor, the production of which could be affected by single nucleotide polymorphisms (SNPs) of IL10 gene. This study was performed to investigate genotype frequencies of IL10 in a group of individuals with RAS.
Methods: Genomic DNA of 60 Iranian patients with RAS were typed for IL10 gene (C/A -1082, C/T -819, and C/A -592), using polymerase chain reaction with sequence-specific primers. The frequency of each allele and genotype was compared to a group of healthy individuals.
Results: A significantly higher frequency of T allele at position -819 (P=0.006) and A allele at position of -592 (P<0.001) were found in the group of patients with RAS, when compared to the controls. IL-10 GA genotype at position -1082 (P=0.007), CA genotype at position -592 (p=0.001), and CT genotype at position -819 (p=0.001) were significantly higher in the RAS patients.
Conclusions: The results of this study suggest that certain SNPs of IL10 gene have an association with predisposition of individuals to RAS. However, further multi-center studies are needed to confirm these findings.
Najafi, Shamsolmolouk
( MSc in Oral Medicine,Dental School,Tehran University Of Medical Sciences,Tehran,Iran, Tehran, , Iran
)
Mohammadzade, Mahsa
( Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, , Iran
)
Bidoki, Alireza
( Molecular Immunology Research Center; and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, , Iran
)
Yousefi, Hila
( Faculty of Dentistry,Azad University of Medical Sciences, Tehran, , Iran
)
Farhadi, Elham
( Hematology Department, School of Allied Medical Science, Tehran University of Medical Sciences, Tehran, , Iran
)
Meighani, Ghasem
( Pediatrics Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, , Iran
)
Rezaei, Nima
( Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, , Iran
; Molecular Immunology Research Center; and Department of Immunology, School of Medicine,Tehran University of Medical Sciences, Tehran, , Iran
)
SESSION INFORMATION
Oral Session
Oral Medicine and Pharmacology Research
12/12/2013