This study was enrolled on parietal bone of rats in order to evaluate the effect of HBMG and autograft on reconstruction of bone defects. The comparison was based on radiographic evaluation by two methods and histopathologic appearances to clarify the mechanism of calcification during new bone formation induced by HBMG and autograft. Regardless of the surgical procedure, one of the aims of the present study was to evaluate the role of radiographic findings in determining the pattern of osteogenesis in parietal bone of rats and the ability to follow –up the process.
Methods:
This was an experimental study was conducted in 12 rats. Data was collected by digital and film-based radiographic observations and microscopic evaluations, carried on 4 groups of rats sacrificed in 7th, 14th, 24th and 60th days after surgery.
Results:
The results showed increasing in new bone formation by time. The new bone was made in defects containing HBMG was significantly more opaque in comparison with autograft. There was no significant difference between two bone substitutes. Interestingly the results of histopathologic findings were the same as digital radiographic appearances.
Conclusions:
Our finding suggested that HBMG has the same effects as autograft and this material could be useful in reconstruction of bone defects in parietal bone of rats(1)Osteoinductivity of HBMG in rats was different from the former studies as they mentioned that bone reconstruction by osteoinductive materials would be initiated from new cartilage and bone formation.(2,3,4)but we did not find any cartilaginous tissue. Digital imaging was superior to film- based techniques for evaluation of bone formation when using HBMG and autograft, because of the ability in picture enhancement and adjustment of exposure factors according to the site of interest.