Methods: After culturing and passaging these cells, samples of iBond (Heraeus, Kuzler), Excite (Ivolar, vivadent), One Step Plus (Bisco) and Clearfil SE Bond (kuraray), were prepared in capillary tubes and inserted in cultures. The positive control group was sterile water and negative control group was normal culture media. Cytotoxic effects were determined after 1, 24 and 148 hours by MTT assay. Statistical analyses were made using Kruskall Wallis and Mann Whitney-U tests.
Results: The optical density of cytotoxicity after 1hr, 24hrs and 148hrs were: (0.191±0.03, 0.270 ±0.14 and 0.183±0.02) for iBond, (0.139±0.01, 0.157± 0.12 and 0.189±0.03) for Excite, (0.137±0.01, 0.164±0.05 and 0.179±0.18) for One Step Plus, (0.147±0.03, 0.210±0.16 and 0.174±0.03) for SE Bond, (0.139±0.01, 0.155±0.02, 0.191±0.07) for negative control and (0.034±0.00, 0.05±0.08, 0.044±0.00) for positive control. None of the bonding agents have a significant difference with the positive control group.
Conclusions: In the condition of this study, it was determined that iBond had the least cytotoxic effects.