Methods: After cleaning, shaping and obturation of sixty single-rooted human anterior teeth, 3mm of root end was resected. Teeth were randomly divided into two experimental groups based on devices used for root-end cavity preparation (n=20) [group 1 (ultrasonic), group 2 (laser)] and two positive (n=10) and negative (n=10) controls. After retrograde cavity preparation, the cavities were filled by CEM cement. Rhodamine B 2% dye penetration method was utilized for leakage evaluation. Comparison of dye penetration among different specimens was done by one-way ANOVA test. Tukey’s post hoc was performed to pair-wise comparison. In this study, P<0.05 was considered statistically significant.
Results: Mean microleakage values in laser, ultrasonic, and positive control groups were 7.12 ± 1.99, 4.30 ± 1.24, and 9.08 ± 1.70, respectively. One-way ANOVA indicated that there was a significant difference between tested groups (P=0.000). Tukey’s post-hoc analysis revealed significant difference in all pair-wise comparisons (P<0.05).
Conclusions: Based on this in-vitro study, microleakage in Ultrasonic-prepared specimens was significantly lower than Laser group.