Methods: Sixty-four human single-canal canine teeth were instrumented and randomly divided into 5 groups containing three experimental groups (n=16) and two negative and positive controls (n=8). In the first group (LGP/AH26) root canal obturation was done by gutta-percha with 2% taper and AH26 sealer. In the second group (SGP/MTA), single gutta-percha cone matching the size of the F3Protaper system file and Pro Root MTA were used for obturation. In the third group specimens (SGP/CEM cement),the same procedure was carried out similar to second group. Teeth were stored at 37 ± 1°C and 100% relative humidity for 7 days. After this period, bacterial leakage apparatus with Enterococcus Faecalis ATCC 29212 species was utilized for leakage assessment. Leakage comparison between experimental groups was done using one-way ANOVA by SPSS 16 statistical software at significancy level of 0.05.
Results: The mean bacterial leakage day in SGP-MTA, SGP-CEM cement, and LGP-AH26 experimental groups was 40.69 ± 11.03, 38.06 ± 9.03, and 33.69 ± 9.39, respectively. Statistical analysis indicate that there was not any significant differences in bacterial leakage between three experimental groups (P=0.436, df=2).
Conclusions: Based on the findings of this study, single cone obturation with well-fitted gutta percha and MTA and CEM cement can be an alternative method for cold lateral compaction technique.