Methods: Twenty four Premolar teeth extracted because of periodontal disease were gathered for this study. Then they were divided in three groups and in each groups the containing agent was applied for 1, 2, 3 and 4 min. Each of the samples were evaluated before and after etching by SEM.
Results:
Dentine amount, Tubular space and mean tubular diameter were our parameters evaluated. Photographies were evaluated by Photoshop and softwares and were:1) In control group there is no relation by time 2) In EDTA group there is a linear and stables relation 3) In Doxycycline group there is a non linear and unstable relation with negative index.
Conclusions: According to our results, in the control group (normal saline) there was no time related relationship seen. There was a stable and liner relationship in the EDTA group.