Methods: This study contains three groups of alginate impressions. The first and the second groups were alginate prepared with 500 and 1000 ppm liquid nano-silver particles. The third one was mixed with water as a control group. Escherichia. Coli, Streptococcus.mutans, and Candida Albicans were used as oral germs in this study. Alginate were prepared following the factory instruction in sterile condition; besides, 24 disks (d=7mm) for each group were prepared. They were placed in culture media inoculated with the indicator strains. After incubation inhibition of the microbial growth was measured, and the results were interpreted.
Results: Findings showed the alginate prepared with water had no zone of inhibition (ZOI) in each qualitative test for three germs included E.coli, streptococcus.mutans and Candida Albicans. These tests for alginates prepared with 500 ppm and 1000 ppm nanocid colloid had a positive reason. An average of three point five mm ZOI in the first group, and four point five mm in the second group were seen.
Conclusions: The results revealed that two groups alginate with antimicrobial solution have a suitable action against these germs and may be used as a dental impression after supportive studies on their physical properties.