Methods: In the calvarial bone of 8 mature White New Zealand male rabbits, four 8 mm defects were created. Each defect was assigned to receive one of the following treatments: Group 1: 0.2mg Nano- HA granule+2ml culture medium; Group 2: 0.2mg Nano-HA+1ml autologous PRGF+2ml acellular culture medium; Group 3: 0.2mg Nano-HA+2ml culture medium containing 100'000 autogenous MSCs; Group 4: 0.2mg Nano-HA+2ml culture medium containing 100'000 autogenous MSCs+1ml autologous PRGF. Kruskal-Wallis analysis was used to detect statistically significant differences between the four treatment groups.
Results: Histomorphometric analysis at 6 and 12 weeks demonstrated a significantly higher bone formation in group 4 (29.45% and 44.55% respectively) (P<0.05). Bone formation in groups 2, 3, and 4 were as follows: 11.35% and 32.53%; 29.10% and 39.74%; 25.82% and 39.11% respectively.
Conclusions: NanoBone with MSC and PRGF seems to be an effective combination for bone regeneration in rabbit calvaria model.