The aim of this study was to prepare a novel bioactive and biodegradable poly (lactide-co-glycolide)/bioactive glass/Hydroxyapatite (PBGHA) nanocomposite coating suitable for improvement of the stem cells attachment.
Methods:
Sol-gel derived 58S bioactive glass (BG) nanoparticles, 50/50wt% poly (lactic acid)/poly (glycolic acid) (PLGA), and hydroxyapatite nanoparticles were used to prepare the coating. The nanoparticle amounts were chosen 10, 15, and 20 weight percent (wt %) in order to determine the optimum amount of nanoparticles that was suitable for prepareing a uniform coating. Bioactivity and degradation of the coating were evaluated. The effect of the nanocomposite coating with an optimum amount of the nanoparticles on the attachment of human adipose-derived stem cells (hASCs) was investigated. Mechanical stability of the coating was studied during intramedullary implantation of coated Kirschner wires (k-wires) into rabbit tibiae.
Results:
Using 10 wt% nanoparticles in the nanocomposite coating could provide a uniform desired coating. Rapid formation of bone-like apatite on the nanocomposite coating was observed. It was degraded considerably after about 60 days. The hASCs showed excellent attachment on the coating. PBGHA coating remained stable on the k-wires with a minimum of 96% of the original coating mass.
Conclusions:
It was concluded that PBGHA nanocomposite coating provides an ideal surface for the stem cells attachment.