COMPARISON OF CYTOTOXIC EFFECTS OF TWO RESIN-BASED SEALERS (AH26 AND 2 SEAL) ON L929 FIBROBLAST CELLS

Objectives: During root canal therapy, the apical area of an obturated root and the nature of materials used for obturation have important roles in an ideal biological response. During the initial steps, irritative potential of these materials may delay or even halt the repair process. For this reason, it would be preferred to use materials that do not irritate or damage apical and periapical tissues. Therefore, these materials must possess high levels of biocompatibility and be non-irritating to surrounding tissues. The aim of this study was to compare cytotoxic effects of two resin –based sealers (in vitro) on L₉₂₉ fibroblast cells.

Methods: This study was carried out using an experimental method. Under aseptic conditions, 20 specimens were tested for each sealer (AH₂₆ and 2 Seal). Monolayer cell cultures were prepared on 24 well plates. After Incubation at C and 5% CO₂-containing humidified air atmosphere, clorometrically by MTT assay was done after 1 hour, 24 hours and 7 days. Optical density was read by ELISA Reader in 570 nm.

Results: Our finding showed that AH₂₆ displayed lower cytotoxicity if the preparation was done using the fresh mix technique. But in the case of four hours after mixing, there was not a significant difference between the two sealers. After 24 hours, AH₂₆ displayed lower cytotoxicity using both techniques, and on the 7^th day, no difference was observed between the two sealers.

Conclusion: The results illustrate that AH₂₆ presented lower cytotoxicity in comparison with 2 Seal. Maximum cytotoxicity is demonstrated when using 2 Seal, and occurs after 24 hours. In fact, this study demonstrated that both sealers, in different times and with different preparation techniques, displayed considerable cytotoxicity. Therefore, it appears that 2 Seal cannot be used as an appropriate alternative to AH₂₆.

Keywords: Citotoxicity effect, AH₂₆, 2 Seal, L₉₂₉ fibroblast cells.