Methods: 95 Instrumented single canal teeth were divided into 4 experimental groups (lateral condensation and AH26, CEM, MTA plus single cone) and 3 control groups (negative control, positive control and single gutta percha cone). A split chamber leakage method using E.Faecalis as a microbial marker was used for evaluation of bacterial microleakage. Bacterial penetration was monitored over a 42-day period. In the case of observing turbidity in lower chamber, microleakage was recorded and control cultures on specific media were performed in order to ensure purity.
Results: At the end of the study period the percent of microleakage was 30% for lateral condensation, 20% for AH26+single cone, 84.2% for CEM+single cone and 60% for MTA+single cone. The only statistically significant difference was observed between the CEM+single cone and lateral condensation groups. (P<0.001)
Conclusion: Considering the specific conditions of this study, the highest resistance to bacterial leakage was achieved by the modified single cone technique using AH26, which was comparable to that of lateral condensation.
Keywords: Modified single cone, Easyfill, Bacterial leakage, Enterococcus Faecalis.