An in vitro study on anti-microbial activity of aqueous and organic extracts of internal shell of walnut against of some oral microbial strain. NOT PRESENTED

Back ground & Aim: Microorganisms are the main etiologic causes of periodontal
diseases and dental caries. Anti-microbial agents with mechanical debridment (as the
main treatment) can help us eliminating microbial factor. Use of therapeutic herbs
because of their natural type has been considered as a strategy.
The aim of this study was to evaluate antimicrobial effect of aquatic and organic
extract of internal shell of walnut against some bacterial strain.
Materials and methods: In this in vitro study, each of the eight strains of bacteria
was cultured in blood agar and Mular Hintone media. Paper disk (with 6mm diameter)
containing aqueous& organic extract were located on this media, and measured
inhibition zone was measured following 24 hours.
To compare effect of aquas extract, data were analyzed statistically by T test.
Results: The final results of measuring the inhibition zone diameters after 24h are
summarized in table 1.as you see, the most and least inhibition zone diameters are in
order related to A.viscuses and Staph.epidermis. Of course, the efficiency of the
organic extract on L. acidophilus, A.viscuses, staph.aureus and strep.sanguis was
more than the efficiency of the aquatic extract .this difference was significant
statistically. The efficiency of the aquatic extract on p.gingivalis and staph.epidermis
was more than organic ones and it was significant statistically. None of the extracts
affected the mouth streps (strep.mutans ,strep.salivaris, strep.sanguis).
Conclusion: In our study, the effects of aquatic and organic anti microbial extracts of
the internal shell of walnut on some strain were examined. The aquatic extract effect
on A.viscuses and Staph. Epidermis was high, on staph.aureus was moderate, on
p.gingivalis, L. acidophilus was low and it didn't have any effect over strep.mutans,
strep.salivaris, strep.sanguis. The effect of the organic extract on Staph.epidermis,
staph.aureus was moderate, on A.viscuses was high,on L. acidophilus, strep.sanguis ,
p.gingivalis was low and on strep.mutans ,strep.salivaris it had no effect .
Key Words: anti-microbial, aqueous, organic, internal shell, walnut