Bioinductance of the Odontoblasts Induced with the Alternating Electric Field

Objectives: The precise recognition of the structure connecting the enamel through the dentine with the dental pulp is a major aspect of dental science. Elucidating it would give us a better understanding of many physiological processes. During embryonic development and during the tooth life the odontoblasts not only participate in the formation of the dentin, but also they take part in the nutrition and regeneration of the dentin. These processes are connected with the replacement of the ions. The aim of the work was to present the influence of the changes of electric field on the bioinductance of the morphotic elements of odontoblasts. Methods: Teeth freshly extracted (for orthodontic reasons) were used. Immediately after extraction (with the use of local anesthesia), low charges of electric current were passed through the teeth. They were fixed in 2% glutardialdehyde in a cacodylate buffer, then cut and their pulps were fixed (as before) and postfixed in 1% osmium tetroxide, dehydrated and embedded in epoxy resin for electron microscope studies. Ultrathin sections were cut using a MTI ultramicrotome and contrasted with uranyl acetate and lead citrate. They were examined and photographed using JEOL electron microscope. The results were compared to the pictures of extracted teeth not treated with the current. Results: The increase of the electric field intensity causes the stimulation of the mitochondria. The flux of the ions in the intercellular junctions is intensive and the water flows into the cytoplasm, forming the vacuoles. The vacuoles are situated in the outlet of odontoblasts processes. The collagen fibers are more numerous. Conclusion: The electric field exerts the influence on the bioinductance of the odontoblats.