IADR Abstract Archives

Disinfection efficacy to control Candida growth on soft denture liners

Introduction: Candida growth on removable dentures may induce a candidiasis and an irritation of the mucosa especially on PMMA-based soft denture liners and to a lesser extend on Silicone liners. The aim of this in vitro study was to test different possibilities to reduce the candida plaque on a silicone soft denture liner with mouthrinses and a disinfectectant.

Material and Method: Cylindrical specimens (diameter 10mm, thickness 2 mm) were made of a soft denture liner (Mucopren soft, Kettenbach, G) and artificially aged (3000 cycles 5°C/55°C H20; 2min). After incubation with Candida albicans (ATCC10231) for 2,5 h the specimen were incubated with Dynexan (Kreussler Pharma, G; 0,1% Chlorhexidin), Plax (Colgate Palmolive, G; 0,3% Triclosan), and Blend-a-dent 2 Phasen Ultra tabs (Procter & Gamble GmbH, G) according to the manufacturers instructions using the appropriate concentration. Incubation with phosphate buffered saline (PBS) served as a control. Living, adherent Candida colonies were quantified using a Luminescence Kit (ViaLight, Cambrex, USA) and the platereader Fluostar Optima (bmg, G). The setup was performed in triplicate using 10 samples and controls per group. Medians and 25/75 percentiles were calculated, statistics: Mann-Whitney U-test, p=0.05.

Results:

Dynexan

Plax

Blend-a-dent 2 Phasen Ultra

PBS (control)

median

643

1095

18

674

25/75-percentile

353/1224

759/1609

5/71

434/955

The medians of the relative fluorescence intensity varied between 18 and 1095. Almost no colonies were found using Blend-a-dent 2 Phasen Ultra. The difference to every other tested group was significant. The mouthrinses did not reduce the Candida amount significantly.

Conclusion: Only the disinfectant tabs seem to be effective to control Candida growth on the soft denture liner.


Continental European and Scandinavian Divisions Meeting
2005 Continental European and Scandinavian Divisions Meeting (Amsterdam, Netherlands)
Amsterdam, Netherlands
2005
528
Scientific Program
  • Brandl, Simon  ( University of Regensburg, Regensburg, N/A, Germany )
  • Groeger, Gerhard  ( University of Regensburg, Regensburg, N/A, Germany )
  • Rosentritt, Martin  ( University of Regensburg, Regensburg, N/A, Germany )
  • Handel, Gerhard  ( University of Regensburg, Regensburg, N/A, Germany )
    • Poster Session
    Prosthodontics Research
    09/17/2005