Material and Method: Cylindrical specimens (diameter 10mm, thickness 2 mm) were made of a soft denture liner (Mucopren soft, Kettenbach, G) and artificially aged (3000 cycles 5°C/55°C H20; 2min). After incubation with Candida albicans (ATCC10231) for 2,5 h the specimen were incubated with Dynexan (Kreussler Pharma, G; 0,1% Chlorhexidin), Plax (Colgate Palmolive, G; 0,3% Triclosan), and Blend-a-dent 2 Phasen Ultra tabs (Procter & Gamble GmbH, G) according to the manufacturers instructions using the appropriate concentration. Incubation with phosphate buffered saline (PBS) served as a control. Living, adherent Candida colonies were quantified using a Luminescence Kit (ViaLight, Cambrex, USA) and the platereader Fluostar Optima (bmg, G). The setup was performed in triplicate using 10 samples and controls per group. Medians and 25/75 percentiles were calculated, statistics: Mann-Whitney U-test, p=0.05.
Results:
| Dynexan | Plax | Blend-a-dent 2 Phasen Ultra | PBS (control) | |
| median | 643 | 1095 | 18 | 674 |
| 25/75-percentile | 353/1224 | 759/1609 | 5/71 | 434/955 |
The medians of the relative fluorescence intensity varied between 18 and 1095. Almost no colonies were found using Blend-a-dent 2 Phasen Ultra. The difference to every other tested group was significant. The mouthrinses did not reduce the Candida amount significantly.
Conclusion: Only the disinfectant tabs seem to be effective to control Candida growth on the soft denture liner.