Alkaline Peroxide Efficiency on Soft Lining Materials Colonized with C.albicans

Objectives: Silicone-based soft lining materials have been found to be more susceptible to Candidal adhesion. Denture hygiene is essential to maintain the serviceability of the denture, and denture cleaners have been suggested for denture disinfection. The purposes of this study were to examine (1) the ability of Candida albicans to adhere to two permanent soft liners and (2) the effectiveness of alkaline peroxide-type denture cleansers in the disinfection of long-term soft lining materials contaminated with Candida albicans. Method: Two different silicone-based soft lining materials have been used in this study (Molloplast B and Luci-sof). For each soft lining materials, seven specimens ( 10mm-10 mm-3 mm in diameter ) have been prepared. Sterile specimens have been contaminated with Candida albicans and immersed in four different denture cleaners (Efferdent, Polident, Steradent, Correga tabs). The reduction in viable, adherent cells have been calculated by comparison with appropriate control specimens that have been treated in same way as test specimens, but without a disinfection regime. Results: There was statistically differences between the soft liners and denture cleansers (p< 0.0001). Adhesion of Candida albicans to Lucisoft soft liner (486 yeast cells/mm²) was statistically greater than the Molloplast B ( 130 yeast cells/mm²) (p< 0.0001). Inhibition was observed for Candida albicans for two tested soft lining materials. (After disinfection Lucisoft soft liner-258 yeast cells/mm², Molloplast B-49 yeast cells/mm²).Conclusion: This study demonstrated that silicone-based soft lining materials exhibited the Candidal colonization. Alkaline peroxide-type denture cleaners have been found effective in the disinfection of silicone-based soft liners contaminated with C.albicans.