G.B.R. using a Phusiline® membrane: a study in rat calvarium

Objectives: The aim of this study is to establish the ability of the bio-absorbable, bio-resorbable and thermoplastic Phusiline® PLA 98 membrane to follow the principles of guided bone regeneration on bone defects created on the rat calvarium. Methods: A batch of 26 male albino Wistar rats was used. After raising the periosteum, two 6 mm standardized round bony lesions were prepared.The lesions were realized on each side of the median suture: one experimental (test), the other control. The Phusiline ® PLA 98 membrane was heated and adapted to the defect in order to go beyond the sides of the defect of at least 2 mm. Then, the periosteum was carefully sutured above the membrane and the soft tissues were replaced and sutured. The animal were sacrificed with an overdose of Nembutal® by groups, either 20 days, 3 months and 6 months after surgery. After fixation and decalcification, the tissue blocks were sectioned and tainted with the Masson’s blue trichromic stain. A quantitative and qualitative analysis of bone regeneration was performed by means of descriptive histology and histometry. Results: Histometrical analysis determines the percentage of bone regeneration obtained spontaneously (control site) or with the membrane (test site). Descriptive histology was carried out on 26 rats. Generally, three main facts emerged from the histological analysis of the Phusiline® PLA 98 membrane: its perfect tissue integrity, its very low resorption rate and its outstanding faculty of maintaining space under the membrane. At the test sites, we could observe very nice areas of new bone formation. A full closure of the trepanning with a thin bone strip can occur. We observed a full closure of seven trepanning test and 5 trepanning control. Conclusions: Osseous regeneration was significantly greater on sites covered by the polylactic membrane