Methods: Cells cultures were derived from alveolar bone chips obtained from a patient with aggressive periodontitis during periodontal surgery and a clinically healthy subject during surgical procedure for crown lengthening. PRP obtained from 1 healthy volunteer was prepared from plasma by centrifugation. Cells were grown in 24-well dishes and on day 2 of quiescence, were treated with 0.1, 1 and 5% of PRP. To determine the effect on cell proliferation, DNA synthesis was estimated by measuring [3H] thymidine incorporation. Collagenase secretion, after a 6-hour treatment with PRP and subsequent 48-hour incubation in serum-free medium, was estimated by gelatin zymography.
Results: The results of this study showed that the addition of 5% of PRP in the affected osteoblastic cells cultures increased significantly (p<0.01) the cell proliferation rate compared to untreated control cells cultures. Furthermore, the addition of PRP seemed to activate significantly the MMP-2 secretion from the affected as well the healthy cells.
Conclusion: PRP promotes the cell proliferation and modulates MMP-2 secretion from the osteoblasts derived from sites affected by aggressive periodontitis. It might be concluded that PRP could serve as a useful adjunct in the periodontal treatment and further studies are required to determine the effective role of PRP on aggressive periodontitis therapeutic approach.