IADR Abstract Archives
Microbiological Evaluation of Single Versus Multiple Visit Regeneration Using MALDI-TOF-Mass-Spectrometry.
Objectives: This study compared single-visit and multiple-visit regeneration protocols microbiologically using matrix-assisted-laser-desorption-ionization-time-of-flight-mass- spectrometry (MALDI-TOF-MS), clinically and radiographically using cone beam computed tomography (CBCT).
Methods: A randomized controlled clinical trial was performed recruiting 35 patients, 8-13 years with traumatized immature permanent teeth showing periapical lesions. They were randomly divided in two groups before induction of bleeding, Biodentine cervical plug application, and sealing. Group (1) (n=5) single-visit and Group (2) (n=11) multiple-visit. Field decontamination and negative control samples were performed before collecting samples using a file. For both groups, the first visit included access-cavity-preparation and first sample collection (A). Minimal instrumentation, irrigation with 20ml 2.5%sodium-hypochlorite, 10ml saline were done for group (2) or further irrigation with 20ml 17%EDTA,10ml saline for group (1). Then sample (B) was collected, bleeding was induced, and the tooth sealed. For group (2) calcium hydroxide was placed for 1-2weeks then removed and irrigation was done with 20ml 17% EDTA, 10ml saline, and finally sample (C) was collected before bleeding induction. Collected dentin-debris were placed in thioglycolate-broth, cultured, and examined by MALDI-TOF-MS. Cases were followed for 12months. Microbial reduction, absence of signs and symptoms, resolution of lesions and root development presented successful microbiological, clinical, and radiographic outcomes, respectively. The outcomes of both groups were compared statistically (p < 0.05).
Results: No statistically significant difference existed between the two protocols in clinical and microbial outcomes. Radiographically, group2 showed statistically significant decrease in lesions, increase in bone density, root development, and intracanal calcification presence. Group1 did not show complete lesion resolution. The clinical trial was prematurely terminated due to the COVID-19 pandemic. The post hoc power was 94.17% regarding decrease in lesion size.
Conclusions: Single-visit-regeneration-protocol showed similar clinical and microbiological outcomes as multiple-visit however, it failed to show complete healing of the periapical lesions.
Methods: A randomized controlled clinical trial was performed recruiting 35 patients, 8-13 years with traumatized immature permanent teeth showing periapical lesions. They were randomly divided in two groups before induction of bleeding, Biodentine cervical plug application, and sealing. Group (1) (n=5) single-visit and Group (2) (n=11) multiple-visit. Field decontamination and negative control samples were performed before collecting samples using a file. For both groups, the first visit included access-cavity-preparation and first sample collection (A). Minimal instrumentation, irrigation with 20ml 2.5%sodium-hypochlorite, 10ml saline were done for group (2) or further irrigation with 20ml 17%EDTA,10ml saline for group (1). Then sample (B) was collected, bleeding was induced, and the tooth sealed. For group (2) calcium hydroxide was placed for 1-2weeks then removed and irrigation was done with 20ml 17% EDTA, 10ml saline, and finally sample (C) was collected before bleeding induction. Collected dentin-debris were placed in thioglycolate-broth, cultured, and examined by MALDI-TOF-MS. Cases were followed for 12months. Microbial reduction, absence of signs and symptoms, resolution of lesions and root development presented successful microbiological, clinical, and radiographic outcomes, respectively. The outcomes of both groups were compared statistically (p < 0.05).
Results: No statistically significant difference existed between the two protocols in clinical and microbial outcomes. Radiographically, group2 showed statistically significant decrease in lesions, increase in bone density, root development, and intracanal calcification presence. Group1 did not show complete lesion resolution. The clinical trial was prematurely terminated due to the COVID-19 pandemic. The post hoc power was 94.17% regarding decrease in lesion size.
Conclusions: Single-visit-regeneration-protocol showed similar clinical and microbiological outcomes as multiple-visit however, it failed to show complete healing of the periapical lesions.