IADR Abstract Archives
Dentinal Tubules Occlusion and Remineralization Potential of Eggshell and Seashell Nanoparticles as Monotherapy and Combined with Nd: YAG Laser
Objectives: There is an interest in developing materials with a bioactive potential that could block the exposed dentinal tubules. This study compared the effects of eggshell and seashell nanoparticles individually or combined with ND:YAG laser on dentinal tubules occlusion and remineralization
Methods: Fifty radicular dentin discs were prepared from freshly extracted human premolars. The smear layer created by cutting was removed using 37% phosphoric acid gel for 15 sec. Discs were randomly divided into five groups according to the applied treatment (n=10 each). Group (A1), received no surface treatment (control), group (A2): Eggshell, group (A3), Seashell, group (A4); Eggshell+ Neodymium-doped Yttrium Aluminum Garnet Laser (Nd:YAG laser), group (A5); Seashell+Nd:YAG laser. Each specimen was evaluated for tubular patency and minerals content before and after each therapy using ESEM-EDXA energy dispersive spectroscopy for assessment of tubules occlusion and remineralization.
Results: ESEM results revealed a statistical significant decrease in the mean percent changes of dentinal tubules number after treatment with the experimental groups compared to the control. The greatest percent decrease recorded with Eggshell combined with Nd:YAG laser group, followed with combined therapy of Seashell and Nd:YAG laser, then monotherapy of Seashell, while the least decrease recorded with Eggshell group. EDXA revealed that, the greatest percentage increase in Ca wt % and Ca/P % was recorded in Eggshell+ Nd:YAG laser, followed by Eggshell only, then Seashell only, then Seashell NPs+ Nd:YAG laser, while the lowest percent increase was recorded in the control.
Conclusions: Both eggshell and seashell nanoparticles are effective in occluding and remineralization of dentinal tubules. The combined treatments with Nd:YAG laser had no benefits when compared to the effect of treatments alone.
Methods: Fifty radicular dentin discs were prepared from freshly extracted human premolars. The smear layer created by cutting was removed using 37% phosphoric acid gel for 15 sec. Discs were randomly divided into five groups according to the applied treatment (n=10 each). Group (A1), received no surface treatment (control), group (A2): Eggshell, group (A3), Seashell, group (A4); Eggshell+ Neodymium-doped Yttrium Aluminum Garnet Laser (Nd:YAG laser), group (A5); Seashell+Nd:YAG laser. Each specimen was evaluated for tubular patency and minerals content before and after each therapy using ESEM-EDXA energy dispersive spectroscopy for assessment of tubules occlusion and remineralization.
Results: ESEM results revealed a statistical significant decrease in the mean percent changes of dentinal tubules number after treatment with the experimental groups compared to the control. The greatest percent decrease recorded with Eggshell combined with Nd:YAG laser group, followed with combined therapy of Seashell and Nd:YAG laser, then monotherapy of Seashell, while the least decrease recorded with Eggshell group. EDXA revealed that, the greatest percentage increase in Ca wt % and Ca/P % was recorded in Eggshell+ Nd:YAG laser, followed by Eggshell only, then Seashell only, then Seashell NPs+ Nd:YAG laser, while the lowest percent increase was recorded in the control.
Conclusions: Both eggshell and seashell nanoparticles are effective in occluding and remineralization of dentinal tubules. The combined treatments with Nd:YAG laser had no benefits when compared to the effect of treatments alone.