IADR Abstract Archives
Dental Pulp Stem Cell Response to Silver Diamine Fluoride: An In Vitro Study
Objectives: To evaluate the effect of SDF on the vitality, gene expression and biomineralization potential of the dental pulp stem cells (DPSCs).
Methods: Mesenchymal stem cells were isolated from the dental pulp of an extracted tooth. Flow cytometry was done to assess their stemness. Two different concentrations of SDF with minimum essential media (0.00001% and 0.0001%) were prepared to treat the DPSCs for up to 7 days. The morphology of the cells was observed by a phase contrast microscope. Cytotoxicity of SDF evaluation was done using a CCK-8 assay. Cell vitality was evaluated with a live/dead assay using a fluorescence microscope. Quantitative evaluation of different gene expression levels (ALP, DSSP, DMP-1, IL-1, IL-6, and IL-8) was done using real-time polymerase chain reaction RT-PCR. The biomineralization potential of DPSC was evaluated by alkaline phosphatase (ALP) assay.
Results: Flow cytometry results showed that the isolated cells expressed the profile of DPSCs. phase contrast microscopy showed the DPSCs’ morphology was affect by the different concentrations of SDF. CCK-8 cytotoxicity assay showed there was an increase in the optical density of the 0.00001% SDF group. Live/dead staining showed that there was a decrease in the number of vital cells in the 0.0001% group. The PCR results show an upregulation in the expression of the tested mineralization and inflammatory genes following the increase in the concentration of SDF in the culture media.
Conclusions: SDF at 0.00001% concentration had a positive effect in terms of vitality and maintain the normal cell morphology, while the 0.0001% did not show the same result. Also, SDF promoted the biomineralization potential by the upregulation of the genes responsible for mineralization.
Methods: Mesenchymal stem cells were isolated from the dental pulp of an extracted tooth. Flow cytometry was done to assess their stemness. Two different concentrations of SDF with minimum essential media (0.00001% and 0.0001%) were prepared to treat the DPSCs for up to 7 days. The morphology of the cells was observed by a phase contrast microscope. Cytotoxicity of SDF evaluation was done using a CCK-8 assay. Cell vitality was evaluated with a live/dead assay using a fluorescence microscope. Quantitative evaluation of different gene expression levels (ALP, DSSP, DMP-1, IL-1, IL-6, and IL-8) was done using real-time polymerase chain reaction RT-PCR. The biomineralization potential of DPSC was evaluated by alkaline phosphatase (ALP) assay.
Results: Flow cytometry results showed that the isolated cells expressed the profile of DPSCs. phase contrast microscopy showed the DPSCs’ morphology was affect by the different concentrations of SDF. CCK-8 cytotoxicity assay showed there was an increase in the optical density of the 0.00001% SDF group. Live/dead staining showed that there was a decrease in the number of vital cells in the 0.0001% group. The PCR results show an upregulation in the expression of the tested mineralization and inflammatory genes following the increase in the concentration of SDF in the culture media.
Conclusions: SDF at 0.00001% concentration had a positive effect in terms of vitality and maintain the normal cell morphology, while the 0.0001% did not show the same result. Also, SDF promoted the biomineralization potential by the upregulation of the genes responsible for mineralization.