IADR Abstract Archives
Antimicrobial potential of Graphine oxide-doped hydroxyapatite nanoparticles
Objectives: Graphine oxide –doped hydroxyapatite nanoparticles are considered a broad spectrum antimicrobial agents against Staphylococcus aureus, Klebseilla pneumonia, E coli, Proteus mirabilis, Pseudomonas aeruginosa and Candida albicans
Methods: Samples of hydroxyapatite incorporated with different concentrations of graphene oxide (1, 5, 10, 25, 50, 100, 200, 300 wt %) was tested against Staphylococcus aureus, Klebseilla pneumonia, E coli, Proteus mirabilis, Pseudomonas aeruginosa and Candida albicans by agar disk diffusion method using Müller-Hinton agar medium according to Coelho et al (2010). The inocula of all microorganisms were prepared from fresh overnight nutrient broth cultures that were incubated at body temrature . The size is standardized using McFarland standard (OD 0.5 at 650 nm).The effect of the material on the stains were calculated in mm which can be mesured .
Results: Results cleared that graphene oxide nanoparticles enhanced the antimicrobial activity of hydroxyapatite against Staphylococcus aureus, Klebseilla pneumonia, E coli, Proteus mirabilis, Pseudomonas aeruginosa and Candida albicans compared to Kanamycin (30 µg) as a positive control. Besides, we noticed that the antimicrobial activity is directly proportional to the concentration of graphene oxide reaching the highest potency at a concentration 300 wt %
Conclusions: They proposed that a three-step antimicrobial mechanism, previously used for carbon nanotubes, is applicable to graphene-based materials. It includes initial cell deposition on graphene-based materials, membrane stress caused by direct contact with sharp nanosheets, and the ensuing superoxide anion-independent oxidation
Methods: Samples of hydroxyapatite incorporated with different concentrations of graphene oxide (1, 5, 10, 25, 50, 100, 200, 300 wt %) was tested against Staphylococcus aureus, Klebseilla pneumonia, E coli, Proteus mirabilis, Pseudomonas aeruginosa and Candida albicans by agar disk diffusion method using Müller-Hinton agar medium according to Coelho et al (2010). The inocula of all microorganisms were prepared from fresh overnight nutrient broth cultures that were incubated at body temrature . The size is standardized using McFarland standard (OD 0.5 at 650 nm).The effect of the material on the stains were calculated in mm which can be mesured .
Results: Results cleared that graphene oxide nanoparticles enhanced the antimicrobial activity of hydroxyapatite against Staphylococcus aureus, Klebseilla pneumonia, E coli, Proteus mirabilis, Pseudomonas aeruginosa and Candida albicans compared to Kanamycin (30 µg) as a positive control. Besides, we noticed that the antimicrobial activity is directly proportional to the concentration of graphene oxide reaching the highest potency at a concentration 300 wt %
Conclusions: They proposed that a three-step antimicrobial mechanism, previously used for carbon nanotubes, is applicable to graphene-based materials. It includes initial cell deposition on graphene-based materials, membrane stress caused by direct contact with sharp nanosheets, and the ensuing superoxide anion-independent oxidation