IADR Abstract Archives
Effect of Chitosan on the Solubility of Root Dentin Treated With Sodium Hypochlorite
Objectives: To evaluate the effect of chitosan (CS) and nanopaticulate chitosan (np-CS) on the solubility of type I collagen present in root dentin treated with sodium hypochlorite (NaOCl).
Methods: In vitro quantitative experimental study, approved by the institutional ethics committee. 36 sticks of root dentin (1.5 x 1.5 x 8 mm) were obtained from 20 healthy human third molars. The samples were randomly distributed into 6 groups (n=6), for immersion in the experimental substances. G1: Control (-), untreated dentin, G2: 1% NaOCl, G3: 1% NaOCl+17% ethylenediaminetetracetic acid (EDTA), G4: 1% NaOCl+ glutaraldehyde (GA), G5: 1% NaOCl+CS, G6: 1% NaOCl+np-CS. To determine the solubility of collagen, the Hydroxyproline Assay kit (Cell Biolabs, INC) was used (San Diego, CA, USA), colorimetric assay by which the amount of total hydroxyproline (HYP) is detected from tissue hydrolysates. The immersion protocol was carried out in the experimental substances for 5 min, then the collagen solubility test was carried out by determining the release of HYP. A Shapiro Wilk test was applied to determine the normal distribution of the data and, subsequently, an ANOVA analysis , and a Tukey post hoc test were performed (p<0.05).
Results: The treatment with NaOCl (G2) increased the degradation of dentinal collagen, marking a significant difference with all the groups analyzed. This difference increased comparatively with groups G4: NaOCl + GA (p= 0.000001) and G6: NaOCl + np-CS (p=0.000001), which identified that both GA as a positive control, and CS and np-CS as experimental solutions, decreased the HYP release. The solubility of collagen promoted by the effect of NaOCl. Thus, evidencing the cross-linking capacity of the CS and np-CS substances.
Conclusions: The biomodification of root dentin is a strategy to improve the mechanical properties of dentin. np-CS, due to its characteristics such as biocompatibility, cross-linking capacity and its availability to obtain since it is a renewable resource, could be an alternative in the treatment of root dentin previously treated with NaOCl.
Methods: In vitro quantitative experimental study, approved by the institutional ethics committee. 36 sticks of root dentin (1.5 x 1.5 x 8 mm) were obtained from 20 healthy human third molars. The samples were randomly distributed into 6 groups (n=6), for immersion in the experimental substances. G1: Control (-), untreated dentin, G2: 1% NaOCl, G3: 1% NaOCl+17% ethylenediaminetetracetic acid (EDTA), G4: 1% NaOCl+ glutaraldehyde (GA), G5: 1% NaOCl+CS, G6: 1% NaOCl+np-CS. To determine the solubility of collagen, the Hydroxyproline Assay kit (Cell Biolabs, INC) was used (San Diego, CA, USA), colorimetric assay by which the amount of total hydroxyproline (HYP) is detected from tissue hydrolysates. The immersion protocol was carried out in the experimental substances for 5 min, then the collagen solubility test was carried out by determining the release of HYP. A Shapiro Wilk test was applied to determine the normal distribution of the data and, subsequently, an ANOVA analysis , and a Tukey post hoc test were performed (p<0.05).
Results: The treatment with NaOCl (G2) increased the degradation of dentinal collagen, marking a significant difference with all the groups analyzed. This difference increased comparatively with groups G4: NaOCl + GA (p= 0.000001) and G6: NaOCl + np-CS (p=0.000001), which identified that both GA as a positive control, and CS and np-CS as experimental solutions, decreased the HYP release. The solubility of collagen promoted by the effect of NaOCl. Thus, evidencing the cross-linking capacity of the CS and np-CS substances.
Conclusions: The biomodification of root dentin is a strategy to improve the mechanical properties of dentin. np-CS, due to its characteristics such as biocompatibility, cross-linking capacity and its availability to obtain since it is a renewable resource, could be an alternative in the treatment of root dentin previously treated with NaOCl.