HPLC determination of BisGMA release from dental resins in Ethanol and his exposition in gingival fibroblasts

Objectives: Develop an analytical method for detection of BisGMA using UV/VIS Spectrophotometry and HPLC in ethanol and acetonitrile mediums, and determine the percentage of cell viability in human fibroblasts of concentrations BISGMA founds.
Methods: Immerse nine pieces of dental composite resin (3M-Z350-A2) in 1.5 ml of ethanol, three pieces for each alcoholic degree; 12 º, 35 º and 50 º, are stored, after 2 hours, 16 hours, 1 day and 22 days are removed the composites and proceeded to measure the solutions in HPLC (fig. 1). After that applying BisGMA concentrations obtained, in cultured human fibroblasts.
Results: In HPLC are obtained concentrations between 1.15 E-5M and 4.6 E-5M BisGMA from a dental composite resin. In the fibroblast cell culture, we can see that the above 4 E-5 M of BisGMA generated lysis in fibroblasts, as well as morphological changes as vacuolization.
Conclusions: BisGMA is soluble in acetonitrile and ethanol, which can be used as a marker for to quantify their release from a dental composite resin, we can conclude that concentration up to 4E-5 M of BisGMA can be potential damage in the cell culture of fibroblast.