The study of muscle tissue engineering with 3D PLGA

Objectives: An understanding of the mechanical and mechano-molecular responses that occur during SD rat myoblast with the cyclic strain in 3D culture is critical for understanding functional orthopedics, which is important for the progress towards producing a tissue-engineered muscle construct. Methods: A 3D culture model has been established in which myoblast contract PLGA scaffold, which is induced by cyclic uni-axial tension. Results: PLGA scaffold, which has the interval porosity of 85% and the pore size of 88~100µm, is a satisfactory scaffold of tissue engineering of myoblast. Induced by cyclic uni-axial tension, the gene expression of Mechano-growth factor (MGF) is monitored during the course of cyclic strain. We found a transient surge in MGF expression on 12h of the developing construct. Conclusion: PLGA scaffold is a satisfactory scaffold of tissue engineering of myoblast. Some differences of function express of the myoblast are existing between the myoblast of 3D culture and those of 2D culture.