Difference of gene expression in dendritic cells presenting anticaries vaccines

[Abstract] Objectives:this study is to analyze: 1.) The difference of gene expression before and after the dendritic cells presenting vaccine antigens, 2.) The difference of gene expression between the dendritic cells presenting untargeted and targeted vaccines.Methods:Use magnetic beads and flow cytometric sorter to isolate dendritic cells from mouse mucosal lymphoid tissues (Peyer's Patches), transfect dendritic cells with target or un-target DNA vaccine, then isolate dendritic cells mRNA and analyse it by mouse gene clips(microarray), using real-time PCR to check the data from microarray. Results: Some gene expression have changed before and after the dendritic cells presenting vaccine antigens, including presenting untargeted and targeted vaccines. Conclusion:From this study, we wish to know more information about mucosal immunity and find someway to enhance efficient of target anti-caries vaccine from this study.