Effects of Curcumin on Invasion Migration of Tongue Squamous Cell

Objectives: Curcumin is a chemical derived from several curcuma species. It gradually becomes a focus because of its anti-cancer effects , but the effects on invasion and migration in tongue squamous cell carcinoma remain less well unterstood. The gelatinases-- matrix metalloproteinase -2 and -9(MMP-2 ,-9) are important prerequisite for tumor invasion and metastasis. In this study, we examined the effect of curcumin on invasion and migration of the tongue squamous cell line Tca8113. Methods: Tca8113 cells were treated with curcumin(0-100µM)for 24h and the conditional medium was collected. The activities of gelatinases in the conditional medium were detected by gelatin zymography. The cell invasion and migration model in vitro was conducted using transwell chamber with or without Matrigel. Using this model, the effects of 50µM curcumin on invasion and migration of Tca8113 were detected.

Results: Curcumin not only reduced the activities of MMP-2 and MMP-9 on a dose-dependent manner, but also suppressed the abilitity of cell invasion and migration significantly (p<0.05 ).

Conclusion: Curcumin can suppress Tca8113 invasion and migration by reducing the activities of MMP-2 and MMP-9.