Methods: By using 96 well plate and test tube for planktonic Candida albicans ,96 wells plates and glass plates combined with 6 well plates for biofilm strains , all the strains were treated with gradient concentration of antifungal drug (from 0.075 to 128 ug/ml) , we isolated the Candida albicans persisters . Reculture the isolated persisters and then test their drug resistance .
Results: The persisters can be isolated in the growth station of both biofilm and plaktonics , but the emergence frequency of persisters of biofilm station is higher than planktonic, about 100 folds. When the isolated persisters were recultured and formed the new biofilm , the characteristics of drug resistance lost .
Conclusion: Similarly to the bacterial persisters , Candida albicans persisters are phenotypic variants of the wild type that arise in a clonal population of genetically identical cells.