Biodistribution of Anti-caries DNA Vaccine pGJA-P/VAX in Mice

Objective: To study the general safety and biodistribution of the targeted anti-caries fusion DNA vaccine pGJA-P/VAX in mice model by Real-time PCR. Methods: pGJA-P/VAX and pVAX1 were extracted and administrated intranasally to mice. At 1d,2d,7d,21d,30d,44d,60d and 74d, organs were collected in this order: testicle, liver, gastrointestine, spleen, kidney,thymus, heart, lung, bronchus, gland under mandibular, muscle around neck, brain, lymph tissue around neck, and nasal mucosa. Tissue DNA were extracted and measured with validated real-time quantitative polymerase chain reaction (QPCR). The results were analysed by software SPSS. Clinical signs were followed up throughout the study and also at the necropsy. Results: Within the early stage after immunization, the plasmid was presented in all of the organs observed, especially in the organs with large quantity circulation. No plasmid was detected in any tissues from the control group immunized with the vector pVAX1. No clinical observations related to the vaccine were found. After statistic analysis of the data resulted from QPCR by SPSS, the amounts of the plasmid were different significantly among times(P<0.05). Conclusions: These results indicated that the DNA vaccine pGJA-P/VAX could be distributed widely to many important organs after intranasally immunization.