Abstract Objective: The purpose of this in vitro study was to evaluate the ability of root canal obturation by ObturaII with System B. Material and Methods:40 human mandibular singal rootcanal premolar teeth were prepared by Tri auto system using NiTi Profile sharper by Crown down and step-beck method.And the teeth were devided into 4 groupsB10 teeth each group. Group A were obturated by cold gutta-percha lateral compaction. Group B were obturated by ObturaII.Group C were obturated by ObturaII with main point .Group D were obturated with ObturaII with SystemB, main cone. AH-Plus sealer were used in the experiment.After obtural materials were completely dryed, all the teeth were put into GOD microleakage model. Acquisited the solution samples from the bottom bottle of the model on the 1st,4th,10th,15th,20th,25th,30th day and check the concentration of glucose in order to measure the microleakage. 5 months after finshing GOD measure,8 teeth of each experimental group were stained by methylee blue and then made into transparent teeth model, the other 2 teeth were observed by scanning electron microscope. Results: There was no singnificant difference among the experimental groups on 1st,4th,7thday(P>0.05). There was singnificant difference among the experimental groups after 10 days(P<0.05).The microleakage of Group D< Group C
Key words: ObturaII,System B,GOD(glucose oxidase), dye penetration, Scanning Electron mircoscopic Correspondence:WeiDong Niu Dalian Medical University(Tel:0411-84720029)