Effects of Cofilin Phosphorylation on the Actin Cytoskeleton Reorganization

Objective: Studies in vitro indicate that many kinds of stimulations can remodel actin cytoskeleton via cofilin phosphorylation. The aim of the research is to study the effects of cofilin on the osteoblasts actin cytoskeleton reorganization induced by fluid shear stress. Methods: The parallel plate flow chamber was designed, and fluid shear stress (12 dyne/cm2) was applied to osteoblasts at different time points by PPFC in vitro. Western blot was used to detect the levels of the cofilin and the phospho-cofilin. Results: A PPFC device was obtained. The level of phospho-cofilin protein was dramatically elevated after loading. Shear stress induced an initial decrease of cofilin at 60 minutes followed by subsequent increases at 120 minutes. Conclusion: The self-made PPFC was simply designed and conveniently operated. On the other hand, cofilin phosphorylation mediates actin cytoskeleton reorganization in the osteoblasts induced by fluid shear stress.