A study of five drinks on Sprague-Dawlye rat's enamel demineralization

Objectives: To evaluate five beverages' demineralization potential on Sprague-Dawlye rat molar enamel. Methods: The erosion relative factor of fifteen drinks were analyzed, including the carbonated beverages, fruit juice, pure milk, higher calcium milk and bottle mineral water, then five were chosen for the study. 36 Sprague-Dawlye rats of 80 days were randomly divided into 6 groups, including Deionization water, Sprite, Yili pure milk, NongFuspring water, President Orange and Masterkong Orange,6 rats per group. All rats had been fed with cariogenic diet for fifteen days, then chosen drinks had fed to rats for 3-month. The amount of drinks was strictly controlled during experiment. When the experiment finished, specimens were prepared with mandibular molar. The Keyes caries scores were calculated by stereomicroscope. Enamel demineralization degree was observed with a cofocal laser scanning microscope. Results: Carbonated drinks and orange drinks had a low pH far away from the critical pH5.5; they had great titratable acidity; the contents of calcium phosphate and fluorine were low. Milks' pH were beyond 5.5,and contained a number of calcium phosphate. Mineral water had high pH ,and had certain inorganic ions. Distilled water had high pH, but hardly contained mineral. The milk' E scores was higher than others; in Ds the scores of Sprite were significantly stronger than others (P<0.05);In Dm and Dx the scores of President Orange and Masterkong Orange were significantly stronger than others (P<0.05). The demineralization degree of the rat's enamel due to acid drinks, including Sprite, President Orange and Masterkong Orange, was higher than that of control group, these results were significantly stronger (P<0.05), Masterkong Orange was the highest. Compared with the acid drinks, the Area and TF of milk and mineral water were lower. Conclusions: All acid drinks have the greatest demineralization potential. Mineral waterand and milk subordinates to that.