Establish of HDPM cells stably-transfected with EDA gene

Objectives: EDA gene, the pathogenic gene for X-linked anhidrotic ectodermal dysplasia, was considered to play a role in early development of mice tooth. However, there were still no systemic study of its role in human tooth development. The purpose of this study is to establish human dental papilla mesenchymal(HDPM) cells stably-transfected with EDA gene so that more studies would be carried on with these cells. Methods: Eukaryonic plasmid pIRES2-EGFP-EDA was constructed. HDPM cells were transfected with the mixture of lipofectamine and the plasmid. The positive cell clones were selected with G418. The stable transfection and expression of EDA in HDPM cells were determined by fluorescence and immunofluorescence. Results: Human dental papilla mesenchymal cells were stably transfected with EDA gene. And EDA could be strongly expressed in the transfected cells though weak expression could also be detected in non-transfected cells. Conclusions: The human dental papilla mesenchymal cells stably-transfected with EDA gene was obtained.