Objectives: The aim of this study was to evaluate the influence of platelet-rich plasma (PRP) on cultured periodontal ligament fibroblasts by using an in vitro model of wound healing. Methods: Platelet-rich plasma (PRP) was isolated by two-step centrifugation. Primary PDLFs were obtained from mongrel normal first premolar. The 4th passage of PDLFs were cultured in 12-well chamber plates. The cells were grown to confluence and in vitro wounds were mechanically created, removing a 8mm diameter cells within a circular area using a sterilized silicon bung. The wells were then incubate for 1, 4, 7, 9 and 11 days and the healing speed of these groups was evaluated. Results: The proliferation and the migration of cells were enhanced by addition of 1%PRP and 10%PRP. Conclusions: PRP appears to exert an influence on cells that is compatible with improving wound healing.
Division: Chinese Division Meeting
Meeting:2005 Chinese Division Meeting (Shanghai, China) Location: Shanghai, China
Year: 2005 Final Presentation ID: Abstract Category|Abstract Category(s):Scientific Groups
Authors
Zhong, Sheng
( Fujian Medical University, Fuzhou, N/A, China
)
Yan, Fu Hua
( Fujian Medical University, Fuzhou, N/A, China
)