Objectives: The aim of this study was to culture human dental pulp stem cells and learn some characters of them.. Methods: Dental pulp tissues from premolar extracted for orthodontic reasons were digested with 3mg/ml collagenase I and 4mg/ml dispase. Cells were implanted in a six-well plate at a low density. Clonogenic cells were isolated and tested for surface markers . They were also induced by DMEM containing 20% fetal bovine serum (Hyclone ,USA), dexamethasone, â sodium glycerophosphate and Vitamine C for 6 weeks .Immunohistochemistry was used to test expression of stro-1,dentinsialoprotein(DSP),Cbfa-1 ,osteopontin(OPN), collagen I, alkaline phosphatase(ALP). RT-PCRwas used to test expression of DSPP, Cbfa-1, BSP . Results: Clone were formed when the cells were planted at a low density. The cells isolated from dental pulp express stro-1,cbfa-1,osteopontin and collagen I and do not express DSP and ALP. But DSP and ALP were positive in induced cells.mRNA of DSPP,Cbfa-1 and BSP were detected in induced cells. After 6 weeks induction. calcium deposite are formed Conclusions: The proliferation ability of the cells isolated from human dental pulp was high .The surface markers of them were similar to mesenchymal stem cells. They differentiated into odontoblast-like cells after induction . They do have some characters similar to mesenchymal stem cells. Other character of them in vitro and in vivo should be studied in future.
Division: Chinese Division Meeting
Meeting:2005 Chinese Division Meeting (Shanghai, China) Location: Shanghai, China
Year: 2005 Final Presentation ID: Abstract Category|Abstract Category(s):Scientific Groups
Authors
Li, Hao Yan
( Shanghai Second Medical University, Shanghai, N/A, China
)
Liang, Jing Ping
( Shanghai Second Medical University, Shanghai, N/A, China
)
Wu, An Ping
( Shanghai Second Medical University, Shanghai, N/A, China
)
Zhang, Xiu Li
( Shanghai Second Medical University, Shanghai, N/A, China
)