Detection and measurement of interleukin-8 in peri-implant crevicular fluid

Objectives: Gingival crevicular fluid (GCF) analysis of various inflammatory mediators has been adopted as a means of providing objective criteria of peri-implant tissue health. In this report, IL-8, GCF volume and clinical parameters in the crevicular fluid were studied.Methods: GCF was sampled from 35 healthy and 35 inflamed sites of implantation patients. IL-8 levels were analysed using ABC-ELISA, clinical parameters were measured, and data analysis was performed using the software package SPSS10.0. Results: Significant differences were observed between healthy implant sites and peri-implantitis sites. GCF volumes has positively correlated with PD, PI, GI and MOB. The total amount of IL-8 was positively correlated with PD and GI. Conclusions: This investigation suggests that GCF volume and IL-8 cytokine may be of value as diagnostic and prognostic markers for peri-implantitis.