Evaluation of modifying collagen matrix with RGD through periodate oxidation

Abstract: Objective: To evaluate the effect of modificating collagen matrices with Arg-Gly-Asp (RGD) peptide through periodate oxidation. Methods: The collagen matrices were modified with RGD peptide, using periodate activation method. The modified collagen matrices and unmodified matrices were characterized by Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), and Electron Spectroscopy for Chemical Analysis (ESCA). Mesenchymal stem cells (MSCs) were used to evaluate the cell compatibility of collagen matrices. Results: In terms of cell growth, the MSCs attached much better on the modified matrix than on the unmodified one. But there was no significant difference between two groups regarding the MSCs proliferation. Compare to the unmodified matrices, the mechanical strength of the modified matrix decreased sharply, and its 3D structure was destroyed. Conclusions: The introducing of specific RGD receptor-mediated adhesion site on matrices obviously enhanced the MSCs adhesion on collagen matrices, but the coupled method of periodate oxidation would likely result in the declination of the mechanical strength of the matrix, as well as the destruction of the matrix structure. This would affect the cell growth on the matrix, and decrease the histocompatibility of the matrices.