Detection of unculturable oral phylotypes associated with periodontitis

Objectives: To detect the relationship of uncultured pathogenic bacteria and periodontitis. Methods: Specific PCR primers were designed for two phylotypes D084 and IO25. Polymerase chain reactions were used to determine the prevalence of these phylotypes in patients with chronic periodontitis, plaque-induced gingivitis and healthy control subjects. Results: The specificity of each primer was validated based on the results of sequence analysis of PCR product. D084 And IO25 were detected in subgingival plaque samples in all three groups. The prevalence of D084 in subgingival plaque in chronic periodontitis (62.9%) and plaque-induced gingivitis (84.6%) was relatively higher than in healthy ones, the difference was statistically significant (P<0.01). The prevalence of IO25 in subgingival plaque in periodontitis patients (71.4%) and plaque-induced gingivitis patients (42.3%) were higher than in healthy ones with statistically significant (P<0.01). The difference between chronic periodontitis group and plaque-induced gingivitis groups was statistically significant (P<0.05). Conclusions: The novel uncultured D084 and IO25 phylotypes could be associated with chronic periodontitis and plaque-induced gingivitis. IO25 maybe play an role in the progress from gingivitis to periodontitis.