Comparison of Galla Chinesis and Sodium Fluoride in Biofilm Model

Objectives: Sodium Fluoride is one of the most effective anticaries chemical compounds, which is widely used in prevention of caries.Meanwhile its side-effects including fluorosis have been reported worldwide. So to find an alternative anticarious method has become the focus of many study. Galla Chinensis, a kind of traditional Chinese medicine, has verified activity of anti-bacteria and anti-virus in vitro. The aim of our study is to compare the antibacterial effect between sodium fluoride and Galla Chinesis in biofilm model and evaluate the feasibility of application of Galla Chinesis in the clinical practice of caries prevention. Methods: A continuous culture biofilm model was constructed in the chemostat, treated with Galla Chinesis and Sodium Fluoride by interval pulse. After a period of continuous cultur, exam the number of bacteria adhering on the HA disc,record the planktonic pH in the flow cell continuously and observe the morphology of the biofilm formed on the HA discs by SEM. Results:1.Galla Chinesis can inhibit the growth of the four oral bacteria in the biofilm just as fluoride.2.Galla Chinesis and fluoride can prevent the descent of the planktonic pH in the flow cell,but Galla Chinesis is less efficient than fluoride.3.The biofilms formed after application of Galla Chinesis and fluoride have less matrix than those applicated with sucrose. Conclusion: Galla Chinensis has definite antibacterial effect, and no obvious difference is found in inhibiting the growth and metabolism of cariogenic bacteria with that of Sodium Fluoride in Chemostat. Therefore, Concerning less side effect of this natural medicine than that of chemical agent, it may be a promising caries prophylaxis agent. Support for this study is provided from Chinese Natural Science Grant (Grant No.30171175)