Methods: The BMSCs were obtained from hybrid dogs and cultured and subcultured. The proliferation and osteogenic differentiation of BMSCs were observed .
Results: The cultured BMSCs comprised a single phenotypic population and displayed a fibroblast-like morphology. Without induction condition, cells changed from spindle-shape to cuboidal and polygonal in cell morphology. With 3 passages process of extensive subcultivation, Alkaline phosphatase (AKP) express and deposition of calcium salt were observed in the culture .
Conclusion: The proliferation and osteogenic differentiation of the BMSCs were observed. The cell can be used as an ideal seed cells for bone tissue engineering. The lower expression of AKP suggests the BMSCs in our culture system are mainly undifferentiated osteoprogenitors.