Pathway of Mechanical Force Signal Transduction on Periodontic Cells

OBJECTIVES:To study the effects of different forces on human periodontal ligament cells (HPDLC)and the pathway of mechanical force signal transduction of cytoskeleton-integrin in vitro. METHODS:cyclic strain force(6cycle/min), fluid shear stress(30r/min) and compressive pressure force (50,100,200kPa)were applied on the third passage of human periodontal ligament cells respectively. Cell reproduction rate and ALP activity, cytoskeleton, integrinb1 were studied using MTT, Coomassie BB and immunohistochemistry staining.RESULTS:the HPDLC were disturbed in different ways,cytoskeleton rearranged, integrinb1 more expressioned by three different of forces.Contrary to fluid shear force and pressure force, circled tension force can stimulate rMSCs and HPDLCs' reproduction and its ALP activity. CONCLUSION:Different forces can cause different effects on HPDLC reproduction, cytoskeleton and integrinb1 expression as well as bone remodeling. This may due to their adapt ability to different forces, as HPDLC used to live in a pressure environment of periodontium. Forces might affect cell proliferation, differentiation and function through the cytoskeleton-integrin pathway of stress signal transduction.