A study of bone formation induced by subperiosteal implantation of guided tissue regeneration collagen membrane and cultured cell complex on calvaria surface in rats
Objective: To study the osteoconductivity of guided tissue regeneration collagen membrane (BME10-X) and periodontal ligament fibroblasts(PDLFs) complex on calvaria intact bone surface of Sprague-Dawley rats. Methods: Sprague-Dawley rat PDLFs were cultured and seeded at a density of 5°Á106cell£¯ml onto the BME-10X® for 10 days in vitro, and then implanted subperiostealy onto cortical bone surface of calvarium in SD rats. Histological examination was performed at 3 and 6 weeks after the PDLFs and BME-10X® complex was implanted. Results: Superior new bone was observed in experimental groups than in controls. Conclusion: PDLFs and BME-10X® complex has the capability of osteoconductivity on the surface of cortical bone. Supported by Foundation of Fujian Province Sciences and Technology Grant No.2001Z021
Division: Chinese Division Meeting
Meeting:2004 Chinese Division Meeting (Wuhan, China) Location: Wuhan, China
Year: 2004 Final Presentation ID:0 Abstract Category|Abstract Category(s):Scientific Groups
Authors
Yan, Fuhua
( School of Stomatology, Fujian Medical University, Fuzhou,Fujian, N/A, China
)
Luo, Kai
( School of Stomatology, Wuhua University, Wuhan, N/A, China
)
Jin, Yan
( College of Stomatology, 4th miliatry Medical University, Xian, N/A, China
)