Cariostatic Activity of Nidus Vespae Within Biofilm Model

Objectives: Within an in vitro biofilm model, the present research aimed to evaluate cariostatic effects of Nidus Vespae on oral biofilm. Methods: A four-organism-bacterial consortium was grown on hydroxyapatite (HA) surfaces in a continuous culture system and exposed to repeated solution pulses. In the positive control group, there was 500 mmol/l sucrose solution pulsed into the flow cell. The experiment group, 4 mg/ml Nidus Vespae extract was pulsed together with the same concentration of sucrose as that of positive control. While in the negative control group, there are distilled water pulsed only. Results: A lower level of colonization of the HA surface was observed in the Nidus Vespae pulsed group compared with sucrose pulsed group, and this was reflected in both the total viable count and biofilm imaging, which has a less compact biofilm. As to the pH responses, the Nidus Vespae solution also helps to the clearance of acid products of carbohydrate metabolism. Conclusions: From the present research, Nidus Vespae may have inhibition effect against biofilm cariogenic shift, and it appears to be a promising source of new agents that may prevent dental caries. This research was supported by Chinese Natural Science Grant 30171175.