ALS Gene mRNA Between Sessile and Planktonic Candida albicans

Objectives: To observe the different mRNA levels of Candida albicans ALS gene family between planktonic and biofilm-grown cells. Methods: ATCC 90038 and a wild strain of Candida albicans , biofilm models in vitro formed on glass slides and 12 well-cell culture plates, after 48 hours incubation, harvested the biofilm-grown cells , half-quantification of ALS1 and ALS4 and ALS3 mRNA based on the amplification by one ¨Cstep RT-PCR. Results: The amounts of ALS1 and ALS4 mRNA of the wild strain in biofilm increase compare with planktonic cells, while ATCC90038 not, and the amount of ALS3 increase in both strains. Conclusions: The members of ALS gene family may play important roles in the course of Candida albicans biofilm formation