The biocompatibility of NC and two bioabsorbable membranes with BMSCs

Objectives:To study the possibility of natural coral (NC) acting as a carrier of bone tissue-engineering ,and compare the physical characters and the biocompatibility with BMSCs of two bioabsorbable barrier membranes, PLGA-NC complex membrane and PLGA membrane, and evaluate the availability of appling them to periodontal guided tissue regeneration (GTR). Methods: Bone marrow stromal cells of primary culture were seeded into porous natural coral particles and two bioabsorbable barrier membranes, PLGA-NC complex membrane and PLGA membrane respectively, the morphological characters of BMSCs in the pores of natural coral particles and the adherent growth condition of cells on the surface of bioabsorbable barrier membranes were observed with phase contrast microscope and scanning electron microscope (SEM). Results: BMSCs could enter the pores of natural coral particles and grew well, associating with bone matrix secreting. There was a good biocompatibility between the two bioabsorbable barrier membranes and BMSCs. Neither of them had cytotoxicity.As to the physical characters, the PLGA-NC complex membrane had more micropores and better plasticity than the PLGA membrane. Conclusions: Natural coral particles can act as the candidate of bone graft material in bone tissue-engineering of periodontal regeneration. PLGA-NC complex membrane was superior to PLGA membrane when applied to GTR.