Study on the Gene Encoding Chimera SBR-CTDA1 Transforming Dunaliella Salina

Objectives: The propose is to study the transformation methods and impact factors of the gene encoding chimera SBR-CT¦¤A1 transforming Dunaliella salina. Methods: ¢Ù To observe the effects of ultrasonic wave of several times and power to Dunaliella salina generation; ¢ÚD. salina was transformed with plasmid pROSB containing the gene encoding chimera SBR-CT¦¤A1 and marker gene(bar) both by ultrasonic wave method and with Agrobacterium binary carrier AT-RSB by Agrobacterium tumefaciens co-cultivation . Results: ¢Ù Dunaliella salina generated well in 55w5min and 88w3min. ¢Ú Total DNA was extracted from random 3 vigorous algal clones by CTAB method in ultrasonic wave method . Results of PCR analyses using a pair of target gene primers preliminarily showed that target gene have been integrated into the genome of D.salina ; results of Agrobacterium tumefaciens co-cultivationwere not well. Conclusions: The transformation system was preliminarily built and transgenic D.salina containing target gene was obtained which may lay foundation for edible vaccines against caries.