IADR Abstract Archives
Angiogenesis and proliferation in immature permanent tooth: exploratory study
Objectives: The pulp and apical papilla can be injured by caries disease or dentoalveolar trauma. To repair the damage, cells are needed to proliferate and migrate to the area of injury. The objective of this exploratory study was to determine the location and presence of cellular proliferation and blood vessels in pulp and apical papilla of immature permanent tooth.
Methods: Pulp and apical papilla were obtained from a third molar with incomplete root formation. It was fixed in 4% PFA for 16 hours. Immunofluorescence was used to determine the presence of Proliferating Cell Nuclear Antigen (PCNA), alpha SMA, CD31 and BrdU.
Results: Immunolocalization of PCNA, CD31, alpha SMA
and BrdU was determined in different areas of the pulp tissue and in the junction zone of the apical papilla with the pulp.
Conclusions: The junction zone between pulp and apical papilla is an area with proliferative cellular activity and abundant blood vessels.
Methods: Pulp and apical papilla were obtained from a third molar with incomplete root formation. It was fixed in 4% PFA for 16 hours. Immunofluorescence was used to determine the presence of Proliferating Cell Nuclear Antigen (PCNA), alpha SMA, CD31 and BrdU.
Results: Immunolocalization of PCNA, CD31, alpha SMA
and BrdU was determined in different areas of the pulp tissue and in the junction zone of the apical papilla with the pulp.
Conclusions: The junction zone between pulp and apical papilla is an area with proliferative cellular activity and abundant blood vessels.